EpiOcular™ was developed by MatTek to create an in vitro (non-animal) alternative to the Draize Rabbit Eye Irritation Test used in the cosmetics, personal care, household products, chemical, pharmaceutical, and biotech industries. EpiOcular™ tissues are metabolically and mitotically active and release cytokines that occur in irritation and inflammation in vivo (IL-6, IL-8, TNFα). This in vitro ocular irritation test is based upon the assessment cytotoxicity following exposure to a test article, at three time points (additional time points may be added). Cytotoxicity is expressed as a decrease in mitochondrial conversion of MTT [(3-4,5-dimethyl thiazole 2-yl) 2,5-diphenyltetrazoliumbromide, into a blue formazan salt that is quantitatively measured after extraction from the 3D EpiOcular™ tissues.
EpiOcular™ has been validated as a Draize Replacement by ECVAM (OECD TG 492 Reconstructed human Cornea-like Epithelium (RhCE) test method) but since its introduction in 1985, EpiOcular™ has been used to determine the ocular irritancy of their products without using animals by many cosmetic, personal care and household product companies.
Test articles can be applied topically allowing for assessment of many types of materials, such as: liquids, solids, semi-solids, pastes, gels, creams and waxes.
MB Research has extensive experience in the use of EpiOcular and in vivo ocular toxicology testing. See abstract.MatTek Corporation.