MatTek EpiDerm™ -Dermal Irritation Test

In Vitro Draize Alternative Tissue Model

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Advanced In Vitro Dermal Irritation Testing

MB Research Labs has been extensive experience using the MatTek EpiDerm and other reconstructed tissue systems for over 20 years. The MatTek in vitro 3D EpiDerm™ model is used to assess for the dermal irritation potential of a test material. The EpiDerm™ tissue model consists of cultured normal, human-derived epidermal keratinocytes that have been to form into a stratified, squamous epithelium which mimics the human epithelium.

MatTek EpiDerm™ is widely used in the following industries/areas:


What is EpiDerm?

EpiDerm™ is 3-dimensional human tissue construct of non-transformed human keratinocytes organized into basal, spinous, and granular layers along a multi-layered stratum corneum with an air-liquid interface. These tissues also exhibit in vivo-like morphological and growth characteristics that allow topical application to the surface of the tissue which mimics the route of human exposure.

3-Dimensional Human Tissue Constructs.  In Vitro Toxicology Assessment.

Viability Testing

Main method endpoint is the Methyl thiazole tetrazolium (MTT) ET50 Tissue Viability Assay. Methyl thiazole tetrazolium (MTT) - Cell viability is measured by dehydrogenase conversion of MTT [(3-4,5-dimethyl thiazole 2-yl) 2,5-diphenyltetrazoliumbromide], which is present in cellular mitochondria, into a blue formazan salt that is quantitatively measured after extraction from tissues by spectrophotometry. The reduction of the viability of tissues exposed to chemicals when compared to negative controls is used to predict the skin irritation potential. Additional endpoints can be derived, including pro-inflammatory mediators, such as IL-1, IL-18, TNF-alpha, etc.


EpiDerm™ has been successfully utilized in alternative in vitro toxicology tests for dermal corrosivity, skin irritation, and phototoxicity.

Applications: product development/efficacy, claims substantiation, safety assessment, and target organ research applications.

Skin Irritation (OECD TG 439)

MB offers GLP safety assessment testing using an extensive list of available protocols that are compliant with standard regulatory requirements.

Skin Corrosion (OECD TG 431)

Regulatory compliant testing with customized study design available.