What is EpiDerm?
EpiDerm™ is 3-dimensional human tissue construct of non-transformed human keratinocytes organized into basal, spinous, and granular
layers along a multi-layered stratum corneum with an air-liquid interface. These tissues also exhibit in vivo-like morphological
and growth characteristics that allow topical application to the surface of the tissue which mimics the route of human exposure.
Main method endpoint is the Methyl thiazole tetrazolium (MTT) ET50 Tissue Viability Assay. Methyl thiazole tetrazolium (MTT) - Cell viability
is measured by dehydrogenase conversion of MTT [(3-4,5-dimethyl thiazole 2-yl) 2,5-diphenyltetrazoliumbromide], which is present in cellular mitochondria,
into a blue formazan salt that is quantitatively measured after extraction from tissues by spectrophotometry. The reduction of the viability of tissues
exposed to chemicals when compared to negative controls is used to predict the skin irritation potential. Additional endpoints can be derived, including
pro-inflammatory mediators, such as IL-1, IL-18, TNF-alpha, etc.
EpiDerm™ has been successfully utilized in alternative in vitro toxicology tests for dermal corrosivity, skin irritation, and phototoxicity.
Applications: product development/efficacy, claims substantiation, safety assessment, and target organ research applications.
MB offers GLP safety assessment testing using an extensive list of available protocols that are compliant with standard regulatory requirements.
Regulatory compliant testing with customized study design available.