MB Research presented the following posters at the 2008 Society of Toxicology Annual Meeting in Seattle. To view and print our posters, please click on the links below. The posters are formatted for Adobe® (*.pdf)
"The Porcine Corneal Opacity and Reversibility Assay is an alternative assay that provides evidence of reversible eye irritancy."(PORCORA_SOT_2008.pdf)
- an ex vivo alternative assay that can assess both potential eye irritation and reversibility using excised porcine corneas. There has been favorable correlation between recovery in the in vivo Draize rabbit eye irritation results and the PorCORA.
"Confocal Microscopy of Reversibility in Excised Porcine Corneas." (Confocal_SOT_2008.pdf)
- The PorCORA measures corneal damage and recovery for extended time periods using excised porcine corneas. Tissue damage is assessed by fluorescein retention in the corneas over time and cell viability is measured by confocal microscopy.
"Development of an Oral Photo-LLNA to Identify Photoallergens ." (Oral_Photo-LLNA_SOT_2008.pdf)
- a cytometry-based local lymph node assay (LLNA) adapted to determine the photoallergenic potential of orally administered compounds in the quinolone and quinaxalin antibiotic groups.
MB Research presented the following posters at the 2007 Society of Toxicology Annual Meeting in Charolette.
"Comparison of in vitro phototoxicity Test Methods: 3T3 NRU PT vs. Enhanced Phototoxicity screening Assay in Reconstituted Skin (EPARS)." (3T3vsEPARS_SOT_2007.pdf)
- a side-by-side comparison between 3T3 Neutral Red Uptake Phototoxicity Test and an in vitro phototoxicity test based on three-dimensional, differentiated human keratinocyte cultures, which can accommodate a wide range of vehicles and allow direct topical application of test substances - the "Enhanced Phototoxicity Assay in Reconstituted Skin (EPARS)".
"Use of An Enhanced Local Lymph Node Assay To Correctly Classify Irritants and False Positive Substances.." (ELLNA_SOT_2007.pdf)
- A major weakness of the local lymph node assay is that irritants can produce increases in stimulation index (SI >3) in manner analogous to true sensitizers or allergens. Herein we use an enhanced LLNA with immunophenotypic endpoints and ear thickness measurements to characterize, identify, and correctly classify additional irritants to distinguish true sensitizers.
MB Research presented the following posters at the 2006 Society of Toxicology Annual Meeting in San Diego.
"Validation of a Non-Radioactive Flow-Cytometry based unscheduled DNA synthesis Assay (FL-UDS)"(FLUDS2_SOT_2006.pdf)
- A non-radioactive in vitro test to identify and characterize genotoxic chemicals. This Flow Cytometry-based UDS is sensitive, more rapid and higher-throughput when compared to the standard radiometric UDS assay.
"Final Validation of a Flow Cytometry-based Local Lymph Node Assay with Immunophenotypic Endpoints (FCLLNA_SOT_2006.pdf)"
- The Local Lymph Node Assay (LLNA) is a non-radioactive alternative to the guinea pig sensitization test used to identify and characterize dermal sensitizers. Accuracy is enhanced by the ability to perform immunophenotypic and surface marker (activation) analysis on the isolated lymph node cells.
"Temporal Stability and Vehicle Effects on a-Hexylcinnamaldehyde Responses as a
Positive Control in a Flow Cytometry-based LLNA
(HCA_SOT_2006.pdf)"
- The temporal variability and vehicle effects in the dermal sensitization response of CBA/J mice to Hexycinnamaldehyde (HCA) administration in a Flow Cytometry-based LLNA (FC-LLNA) were analyzed over a six-year period (1999-2005) in a variety of vehicles.
"Etiology of 8-Methoxypsoralen Phototoxicity in Mouse Skin is Dependent on Vehicle, Exposure Time and Administration Site. (8-MOP_Photo_SOT_2006.pdf)"
- The effects of dermal exposure of 8-Methoxypsoralen (8-MOP) plus UVA light on different mice stains, comparing dosing site, time post-dosing, and solvent vehicle.
MB Research presented the following posters at the 2005 Society of Toxicology Annual Meeting in New Orleans.
"Comparison of Alternative Toxicity Test Methods for Ocular Irritation using the Avian Chorioallantoic Membrane: HETCAM vs. CAMVA" (HETCAM_SOT_2005.pdf) - 2.04 MB
- HET-CAM and the Chorioallantoic Membrane Vascular Assay (CAMVA), are alternative toxicology assays to the Draize Test, an animal-based ocular irritation test.
"Validation of a Flow Cytometry-based Photo-Local Lymph Node Assay (Photo-LLNA) for the Identification and Characterization of Photo-Allergens" (PLLNA_SOT_2005.pdf) - 2.11 MB
- A sensitive, non-radiometric flow cytometry-based method to assess lymph node cell (LNC) proliferation, using a solar simulator to photo-activate test chemicals. The Photo-LLNA accurately identifies and resolves true photosensitizers from false positive irritants, sensitizers, and irritants.
MB Research presented the following posters at the 2004 American College of Toxicology Meeting in Palm Springs California.
"Comparison of CAM Assays: HET-CAM vs. CAMVA"
"EPARS: An In Vitro Phototoxicity Test Using Epidermal Tissue Models" (EPARS_SOT_2004.pdf) - 2216KB
- An improvement on existing photo-cytotoxicity tests by (1) accommodating the topical application of test articles and (2) using a primary human cell-based tissue with a morphology that approximates the 3-dimensional characteristics of human skin.
"A Non-Animal Carcinogenicity Test: The In Ovo Carcinogenicity Assay (IOCA)" (IOCA_SOT_2004.pdf) - 2455KB
- Compared to the standard Rodent Chronic Bioassay (RCB), IOCA accurately screens potential carcinogens with decreased expense, space, assay duration, animal usage and human exposure.
"Application of a Modified LLNA to Petroleum-based Products: Dermal Sensitization Potential of Calcium Long-chain Alkylbenzene Sulfonates" (Lubrizol_SOT_2004.pdf) - 1733KB
- An example of the enhanced LLNA, using %B220 expression and B:T cell ratios in combination with mouse ear swelling measurements, which has the potential to discriminate between irritant and allergic responses.
