Alternative Assay Differentiates EU R36/R41, GHS Category I/II and EPA Category I/II

• Recently, we developed the Porcine Corneal Opacity Reversibility Assay (PorCORA) assay, an alternative ocular irritation reversibility assay.
  
• In a 32 compound validation, PorCORA correctly predicted ocular reversibility (88%), EU: R41 (91%), GHS: Category 1 (91%), and EPA: Category I (88%).
  
• PorCORA measures corneal damage and recovery over a 21-day testing period using porcine corneas excised from waste abattoir eyes.
  
• PorCORA is the only non-animal test system able to predict ocular hazard classifications and is currently undergoing regulatory consideration.

MB Research has been conducting in vivo ocular toxicology testing since 1972 and has conducted over 9,700 rabbit eye studies.
We have pioneered ocular alternatives such as the Chorioallantoic Membrane Vascular Assay (CAMVA) and the Bovine Corneal Opacity & Permeability (BCOP) Assay and have conducted over 5,600 BCOPs since 1989.

PorCORA was developed and validated by MB Research and was funded by two Society of Toxicology Colgate-Palmolive Alternative Research Grants.

Publications

• Ocular irritation reversibility assessment for personal care products using a porcine corneal culture assay.
  Donahue DA, Avalos J, Kaufman LE, Simion FA, Cerven DR. Toxicol In Vitro. 2010 Dec 21.
• Novel cultured porcine corneal irritancy assay with reversibility endpoint.
  Piehl M, Gilotti A, Donovan A, DeGeorge G, Cerven D. Toxicol In Vitro. 2010 Feb;24(1):231-9.

Related Links

• ROBatt - Replacement Ocular Battery
• CAMVA - Chorioallantoic Membrane Vascular Assay
• BCOP - Bovine Cornea Opacity/Permeability Test
• MatTek EpiOcular™