In Vitro Toxicology Testing

Effective & Affordable Non-Animal In Vitro Toxicity Testing Alternatives

In Vitro Toxicology Testing Services

Cytotoxicity

  • Cosmetics, Pharmaceutical, Chemicals & Household Products.
  • Multiple Cell lines Available
  • GLP and Non-GLP Screening Protocols
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Phototoxicity Testing

  • In Vitro 3T3 Neutral Red Uptake Phototoxicity Test
  • GLP Regulatory and Non-GLP Screening Protocols
  • Monolayer & 3D Tissue Models Available
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In Vitro Toxicology

  • In Vitro Toxicity Alternatives to Animal Testing.
  • Cost Saving Screening Testing
  • Pharmaceutical Efficacy Testing
  • Discount Pricing
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MB Research Labs specializes in predictive, 3D Tissue and cell-based in vitro toxicity assays using primary cells from many different tissues and organs. We utilize many proprietary GLP assays to allow for use in nearly all levels of drug and product development from early stage screening to pre-clinical in vivo toxicity studies.

MB Research has been a pioneer in the advancement of the 3Rs: Replace, Reduce and Refine since the early 90s. We have assays that can help provide the predictive information earlier in the development phase, reducing overall costs and aids in getting your products on the market more quickly.

45yrs Experience in Toxicology Testing

Your Trusted In Vitro Toxicity Testing Partner.

Call for Quote or for more information about your in vitro toxicity testing needs:US 1-215-536-4110 Or Contact MB.

NEW - In Vitro Sensitization ITS

MB Research has developed a non-animal Integrated Testing Strategy (ITS) for chemical-induced contact hypersensitivity (skin sensitization). This ITS provides a completely non-animal alternative to traditional testing methods. Together, our three assays address three key events on the skin sensitization adverse outcome pathway (AOP).

Adverse Outcome Pathway (AOP)

Assays for In Vitro Sensitization

Direct Peptide Reactivity Assay (DPRA)

  • Accepted in vitro assay for dermal sensitization screening by ECVAM
  • Measures molecular reaction of specific peptides to possible sensitizers

In Vitro Sensitization Screening Assay (IVSA)

  • Using the 3D Reconstructed Human Epidermis protocol currently under review by ECVAM
  • Measures IL18 production in epidermal keratinocytes
  • For more information, see IVSA.

Human Cell Line Activation Test (h-CLAT)

  • Examines dendritic cell activation by quantifying changes in the expression of cell surface markers (CD86 and CD54) in the human leukemia cell line THP-1

Toxicology Test Methods - In Vitro and Alternative


In Vitro and Alternative Ocular Irritation Assays


MatTek EpiOcular™, SkinEthic corneocyte models

  • These models mimic characteristics of the epithelium of the eye
  • Unique properties of 3D models allow test articles to be applied topically, allowing testing of solids, organics, and insoluble materials

  • Uses fertilized chicken eggs
  • INVITTOX PROTOCOL Number 47

  • Uses excised bovine corneas normally discarded
  • Measures two endpoints; 1) changes in opacity, reflecting protein denaturation and corneal injury, and 2) fluorescein permeability reflecting damage to corneal epithelium
  • Optional histological endpoint is available (H & E staining)
  • Measurement of pro-inflammatory mediators in liberated corneal cells (by flow cytometry or ELISA)

  • Uses excised porcine cornea normally discarded
  • Corneas maintained in culture for up to 21 Days
  • Measures "Days to Recover" after exposure to possible Irritants
  • Allows discrimination between Severe Irritant and Ocular Corrosive

  • Uses fertilized chicken eggs on embryonic day 10 or 14 of development
  • Measures hemodynamic effects, injury, and anti-angiogenic effects to the membrane microvasculature
  • Especially useful for alcohol-containing formulations

  • Uses tiered testing strategy of alternative ocular irritation assays (CAMVA, BCOP, PorCORA, PorFocal) to determine regulatory classification of a test material without the use of live animals.
  • Currently Under Development

MB Research uses normal human primary epidermal keratinocytes or fibroblasts (or mouse keratinocytes and fibroblasts)
  • Cells are representative of the eye and skin epithelium
  • Human cells are derived from neonatal, adolescent, or aged donors
  • Cytokine expression or release (LDH, PGE-2, interleukins) can be measured
  • Low serum or serum free protocol options are available

  • Using primary human cell or mouse cell lines in serum-free or low serum media
  • Rate of proliferation or apoptosis is measured by vital dye uptake (MTT or neutral red) or by BrdU incorporation and flow cytometry

In Vitro Dermal Irritation Assays

The 3D models available at MB Research (MatTek EpiDerm™, SkinEthic systems and CellSystems EpiCS®) allow the application of the test material directly to the "skin" surface and permit the assessment of dermal toxicity via a wide variety of parameters.

  • Cytotoxicity via MTT, neutral red viability assay, or flow cytometry
  • Histological examination (H & E) of the epidermal layers, including the stratum corneum
  • Measurement of inflammatory mediator and lytokine release (PGE-2, nitric oxide, TNFa, IL1a, and other interleukins)
  • Dissolution of the tissue equivalent into a single cell suspension, with subsequent flow cytometric analysis allows evaluation of gene expression and peroxide production (antioxidant status of cells)

In Vitro Dermal Corrosivity Assays

  • An accepted alternative to the Draize rabbit corrosivity test that recently has been validated by ICCVAM for use for DOT, FHSA, CPSC, EPA and FDA testing
  • Allows Packing Group assignment of test materials
  • Can be used to compare the relative corrosivity of materials within Packing Groups for product development purposes. See www.corrositex.com for more information.

  • Validated by ECVAM and ICCVAM as an in vitro alternative corrosivity test based on human skin cells
  • Allows testing of material not compatible with Corrositex™


In Vitro Phototoxicity Assays

  • With 3T3 mouse fibroblast cells via the EC/COLIPA validated protocol. See www.3t3nru.com for more information.
  • Using the MatTek EpiDerm™ protocol currently under review by ECVAM
  • Human keratinocytes or fibroblasts


Cytotoxicity Assays

The 3D models available at MB Research (MatTek EpiDerm™, SkinEthic systems and CellSystems EpiCS®) allow the application of the test material directly to the "skin" surface and permit the assessment of dermal toxicity via a wide variety of parameters.

  • Cytotoxicity via MTT, neutral red viability assay, or flow cytometry
  • Histological examination (H & E) of the epidermal layers, including the stratum corneum
  • Measurement of inflammatory mediator and lytokine release (PGE-2, nitric oxide, TNFa, IL1a, and other interleukins)
  • Dissolution of the tissue equivalent into a single cell suspension, with subsequent flow cytometric analysis allows evaluation of gene expression and peroxide production (antioxidant status of cells)

MB Research also performs other in vitro and alternative assays. Please refer to our Capabilities List or feel free to contact a Client Services Representative for assistance.

FEATURED: Cosmetic Safety Testing Roadmap to Regulatory Approval in North America, Europe and Japan

Bennett J. Varsho1, Erwin L. Roggen2, Bart De Wever3, George L. DeGeorge1

1MB Research Laboratories, Spinnerstown, PA, USA
23RsMC, Copenhagen, Denmark
3ALTEXA Development, Monte Carlo, Monaco

The cosmetics industry is a robust business, achieving greater than $250 billion in worldwide sales in 2013. Although major recognizable brands comprise the largest fraction of this total, there are thousands of small companies that operate in the same markets. While the industry association International Cooperation on Cosmetic Regulation (ICCR) seeks long-term harmonization of testing requirements, currently unaligned governmental agencies in different countries make navigation of safety requirements daunting for major manufacturers and Kafkaesque for smaller firms. While also laudable, the development, validation, approval and implementation of new non-animal safety assays often make the path to cosmetic approval seem more formidable than ever. To aid in navigation of the kaleidoscopic variables of cosmetic ingredient and final cosmetic formulation safety testing, the authors have developed a roadmap to safety assessment, focusing on requirements of the United States, the European Union, Japan and Canada.

In Vitro Toxicology Links:

Solving the In Vitro Toxicology Puzzle

MB Research Labs works closely with other leaders in the field of in vitro toxicology and is dedicated to advancing techniques that reduce the use of live animals in Toxicological Research. Below are several related sources that are helpful in developing and validating novel in vitro toxicology testing methods.