MatTek EpiOcular™ -Eye Irritation Test

In Vitro Draize Alternative Tissue Model

EpiOcular™ is currently under validation as a Draize Replacement by ECVAM but since its introduction in 1985, EpiOcular™ has been used to determine the ocular irritancy of their products without using animals by many cosmetic, personal care and household product companies.

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MB Research Labs performs the MatTek in vitro 3D EpiOcular™ model for ocular irritation assessment. The EpiOcular™ tissue model consists of cultured normal, human-derived epidermal keratinocytes that have been to form into a stratified, squamous epithelium which mimics the human corneal epithelium.
3D Tissue Model

EpiOcular™ was developed by MatTek to create an in vitro (non-animal) alternative to the Draize Rabbit Eye Irritation Test used in the cosmetics, personal care, household products, chemical, pharmaceutical, and biotech industries. EpiOcular™ tissues are metabolically and mitotically active and release cytokines that occur in irritation and inflammation in vivo (IL-6, IL-8, TNF╬▒). This in vitro ocular irritation test is based upon the assessment cytotoxicity following exposure to a test article, at three time points (additional time points may be added). Cytotoxicity is expressed as a decrease in mitochondrial conversion of MTT [(3-4,5-dimethyl thiazole 2-yl) 2,5-diphenyltetrazoliumbromide, into a blue formazan salt that is quantitatively measured after extraction from the 3D EpiOcular™ tissues.

Test articles can be applied topically allowing for assessment of many types of materials, such as: liquids, solids, semi-solids, pastes, gels, creams and waxes.

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Applications:

  • Product Development/Efficacy
  • Claims Substantiation
  • Safety Assessment
  • Target Organ Research

MB Research has extensive experience in the use of EpiOcular and in vivo ocular toxicology testing. See abstract.

MB Research is a MatTek-approved expert laboratory for utilizing their 3D tissue equivalent models for in vitro toxicity studies.EpiDerm™ and EpiOcular™

EpiDerm™ and EpiOcular™ are trademarks of MatTek Corporation.