MatTek EpiOcular™ -Eye Irritation Test

In Vitro Draize Alternative Tissue Model

EpiOcular™ is currently under validation as a Draize Replacement by ECVAM but since its introduction in 1985, EpiOcular™ has been used to determine the ocular irritancy of their products without using animals by many cosmetic, personal care and household product companies.

Contact for Quotation Now
MB Research Labs performs the MatTek in vitro 3D EpiOcular™ model for ocular irritation assessment. The EpiOcular™ tissue model consists of cultured normal, human-derived epidermal keratinocytes that have been to form into a stratified, squamous epithelium which mimics the human corneal epithelium.
3D Tissue Model

EpiOcular™ was developed by MatTek to create an in vitro (non-animal) alternative to the Draize Rabbit Eye Irritation Test used in the cosmetics, personal care, household products, chemical, pharmaceutical, and biotech industries. EpiOcular™ tissues are metabolically and mitotically active and release cytokines that occur in irritation and inflammation in vivo (IL-6, IL-8, TNF╬▒). This in vitro ocular irritation test is based upon the assessment cytotoxicity following exposure to a test article, at three time points (additional time points may be added). Cytotoxicity is expressed as a decrease in mitochondrial conversion of MTT [(3-4,5-dimethyl thiazole 2-yl) 2,5-diphenyltetrazoliumbromide, into a blue formazan salt that is quantitatively measured after extraction from the 3D EpiOcular™ tissues.

Test articles can be applied topically allowing for assessment of many types of materials, such as: liquids, solids, semi-solids, pastes, gels, creams and waxes.

Request for More Info


  • Product Development/Efficacy
  • Claims Substantiation
  • Safety Assessment
  • Target Organ Research

MB Research has extensive experience in the use of EpiOcular and in vivo ocular toxicology testing. See abstract.

MB Research is a MatTek-approved expert laboratory for utilizing their 3D tissue equivalent models for in vitro toxicity studies.EpiDerm™ and EpiOcular™

EpiDerm™ and EpiOcular™ are trademarks of MatTek Corporation.