EpiOcular™ is currently under validation as a Draize Replacement by ECVAM but since its introduction in 1985, EpiOcular™ has been used to determine the ocular irritancy of their products without using animals by many cosmetic, personal care and household product companies.
EpiOcular™ was developed by MatTek to create an in vitro (non-animal) alternative to the Draize Rabbit Eye Irritation Test used in the cosmetics, personal care, household products, chemical, pharmaceutical, and biotech industries. EpiOcular™ tissues are metabolically and mitotically active and release cytokines that occur in irritation and inflammation in vivo (IL-6, IL-8, TNFα). This in vitro ocular irritation test is based upon the assessment cytotoxicity following exposure to a test article, at three time points (additional time points may be added). Cytotoxicity is expressed as a decrease in mitochondrial conversion of MTT [(3-4,5-dimethyl thiazole 2-yl) 2,5-diphenyltetrazoliumbromide, into a blue formazan salt that is quantitatively measured after extraction from the 3D EpiOcular™ tissues.
Test articles can be applied topically allowing for assessment of many types of materials, such as: liquids, solids, semi-solids, pastes, gels, creams and waxes.
MB Research has extensive experience in the use of EpiOcular and in vivo ocular toxicology testing. See abstract.MatTek Corporation.