MatTek EpiOcular™ -Eye Irritation Test

In Vitro Draize Alternative Tissue Model

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Advanced In Vitro Ocular Irritation Testing

MB Research Labs performs the MatTek in vitro 3D EpiOcular™ model for ocular irritation assessment. The EpiOcular™ tissue model consists of cultured normal, human-derived epidermal keratinocytes that have been to form into a stratified, squamous epithelium which mimics the human corneal epithelium.
3D Tissue Model

Background Information

EpiOcular™ was developed by MatTek to create an in vitro (non-animal) alternative to the Draize Rabbit Eye Irritation Test used in the cosmetics, personal care, household products, chemical, pharmaceutical, and biotech industries. EpiOcular™ tissues are metabolically and mitotically active and release cytokines that occur in irritation and inflammation in vivo (IL-6, IL-8, TNFα). This in vitro ocular irritation test is based upon the assessment cytotoxicity following exposure to a test article, at three time points (additional time points may be added). Cytotoxicity is expressed as a decrease in mitochondrial conversion of MTT [(3-4,5-dimethyl thiazole 2-yl) 2,5-diphenyltetrazoliumbromide, into a blue formazan salt that is quantitatively measured after extraction from the 3D EpiOcular™ tissues.

EpiOcular™ is currently under validation as a Draize Replacement by ECVAM but since its introduction in 1985, EpiOcular™ has been used to determine the ocular irritancy of their products without using animals by many cosmetic, personal care and household product companies.

Test articles can be applied topically allowing for assessment of many types of materials, such as: liquids, solids, semi-solids, pastes, gels, creams and waxes.

Toxicological Applications

  • Product Development/Efficacy
  • Claims Substantiation
  • Safety Assessment
  • Target Organ Research

MB Research has extensive experience in the use of EpiOcular and in vivo ocular toxicology testing. See abstract.

MB Research is a MatTek-approved expert laboratory for utilizing their 3D tissue equivalent models for in vitro toxicity studies.EpiDerm™ and EpiOcular™

EpiDerm™ and EpiOcular™ are trademarks of MatTek Corporation.

Other In vitro/Alternative Eye Irritation Tests

OptiSafe™ Eye Irritation Test

  • OptiSafe™ In Vitro Eye Irritation Test
  • An in vitro test method in which a test substance is applied to a semi-permeable membrane. Damage to macromolecules in the membrane is measured to assess the test substance’s potential to cause eye irritation.
  • Can be used to determine the irritation potential of cosmetics, creams, and a wide variety of consumer products. Results are presented as GHS, EPA classifications, an ocular irritation score and class

Porcine Cornea Reversibility Assay (PorCORA)

  • Uses excised porcine cornea normally discarded
  • Corneas maintained in culture for up to 21 Days
  • Measures "Days to Recover" after exposure to possible Irritants
  • Allows discrimination between Severe Irritant and Ocular Corrosive

Chorioallantoic Membrane Vascular Assay (CAMVA)

  • Uses fertilized chicken eggs on embryonic day 10 or 14 of development
  • Measures hemodynamic effects, injury, and anti-angiogenic effects to the membrane microvasculature
  • Especially useful for alcohol-containing formulations

HET-CAM - Hen's Egg Test - Chorioallantoic Membrane (HET-CAM)

  • Uses fertilized chicken eggs
  • INVITTOX PROTOCOL Number 47

Bovine Corneal Opacity and Permeability Assay (BCOP)

  • Uses excised bovine corneas normally discarded
  • Measures two endpoints; 1) changes in opacity, reflecting protein denaturation and corneal injury, and 2) fluorescein permeability reflecting damage to corneal epithelium
  • Optional histological endpoint is available (H & E staining)
  • Measurement of pro-inflammatory mediators in liberated corneal cells (by flow cytometry or ELISA)

Replacement Ocular Battery (ROBatt)

  • Uses tiered testing strategy of alternative ocular irritation assays (CAMVA, BCOP, PorCORA, PorFocal) to determine regulatory classification of a test material without the use of live animals.
  • Currently Under Development