Direct Peptide Reactivity Assay (DPRA)
The Direct Peptide Reactivity Assay (DPRA) is an in chemico method used to predict epidermal protein binding. Binding of epidermal proteins is the molecular initiating event on the Adverse Outcome Pathway. The DPRA uses HPLC to measure the depletion of synthetic peptides in solution following exposure to test chemicals.
Regulatory Acceptance - OECD 442C
OECD Test Guideline 442C is accepted for use. DPRA testing is considered validated by EURL-ECVAM, but should be used as part of an intergrated approach to testing and assessment (IATA) for discrimination between sensitizers and non-sensitizers for hazard classification and labelling.