Cosmetics and Ingredients Safety Evaluation Services

Health and Beauty Products, Cosmetics and Cosmetic Ingredients

MB Research, a leading cosmetic safety testing laboratory, conducts Good Laboratory Practice (GLP) compliant assays for companies around the globe.

Your Alternative and In Vitro Toxicology Testing Specialists.

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Cosmetic, Beauty, Healthcare Products Testing

MB Research has been conducting product safety assessments for the cosmetics, personal care, chemical and pharmaceutical industries for over 40 years and is often sought out by Cosmetics Industry Leaders to ensure the safety their ingredients and aid in the rapid development of products.

MB Research Scientists develop and conduct in vitro and alternative toxicology testing assays such as nitric oxide production, melanin transfer, cell proliferation, apoptosis, ocular irritation, dermal irritation, phototoxicity, angiogenesis / antiangiogenesis and corrosivity.

We are a leader in the use and development of In Vitro and Alternative Toxicology Tests and continue to introduce new testing methods regularly.

More information about specialized research projects is available by calling us directly and speaking with Client Services.

Cosmetic, Beauty and Healthcare product industries are subject to global regulatory requirements such as the EU Cosmetics Directive (76/768/EEC) in response to growing concern over the safety of cosmetic products and their ingredients.

In Vitro Sensitization for Cosmetics

MB offers an multi-tiered Integrated Testing Strategy following specialized dermal sensitization Adverse Outcome Pathway (AOP).

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Phototoxicity Testing

  • In Vitro 3T3 Neutral Red Uptake Phototoxicity Test
  • OECD 432 protocol
  • 3D Human Tissue Model
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In Vitro Toxicology

MB Research is a leader is the use and development of in vitro toxicology testing. Standard assays are available for eye & skin irritation.

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FEATURED: Cosmetic Safety Testing Roadmap to Regulatory Approval in North America, Europe and Japan

Bennett J. Varsho1, Erwin L. Roggen2, Bart De Wever3, George L. DeGeorge1

1MB Research Laboratories, Spinnerstown, PA, USA
23RsMC, Copenhagen, Denmark
3ALTEXA Development, Monte Carlo, Monaco

The cosmetics industry is a robust business, achieving greater than $250 billion in worldwide sales in 2013. Although major recognizable brands comprise the largest fraction of this total, there are thousands of small companies that operate in the same markets. While the industry association International Cooperation on Cosmetic Regulation (ICCR) seeks long-term harmonization of testing requirements, currently unaligned governmental agencies in different countries make navigation of safety requirements daunting for major manufacturers and Kafkaesque for smaller firms. While also laudable, the development, validation, approval and implementation of new non-animal safety assays often make the path to cosmetic approval seem more formidable than ever. To aid in navigation of the kaleidoscopic variables of cosmetic ingredient and final cosmetic formulation safety testing, the authors have developed a roadmap to safety assessment, focusing on requirements of the United States, the European Union, Japan and Canada.

Current available test models are listed below:

In Vitro Toxicology Tests for Cosmetics

Alternative Toxicology Tests

Photobiology/Phototoxicology Studies

Specialized Studies

  • Melanin Transfer
  • Skin Whitening
  • 3-D Human Tissue Constructs Testing

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MB Research Labs - Cosmetics, Chemicals, Consumer Products, Pharmaceuticals
45yrs Experience in Toxicology Testing

Your Trusted In Vitro Toxicity Testing Partner.

Call for Quote or for more information about your in vitro toxicity testing needs:US 1-215-536-4110 Or Contact MB.

Ocular Irritation Assays


Three Dimensional Human Ocular Tissue Equivalent Systems

MatTek EpiOcular™, SkinEthic corneocyte models

  • These models mimic characteristics of the epithelium of the eye
  • Unique properties of 3D models allow test articles to be applied topically, allowing testing of solids, organics, and insoluble materials

HET-CAM - Hen's Egg Test - Chorioallantoic Membrane (HET-CAM)

  • Uses fertilized chicken eggs
  • INVITTOX PROTOCOL Number 47

Chorioallantoic Membrane Vascular Assay (CAMVA)

  • Uses fertilized chicken eggs on embryonic day 10 or 14 of development
  • Measures hemodynamic effects, injury, and anti-angiogenic effects to the membrane microvasculature
  • Especially useful for alcohol-containing formulations

Bovine Corneal Opacity and Permeability Assay (BCOP)

  • Uses excised bovine corneas that are normally discarded as waste in food production
  • Measures two endpoints; 1) changes in opacity, reflecting protein denaturation and corneal injury, and 2) fluorescent dye permeability reflecting damage to corneal epithelium
  • Optional histological endpoint is available (H & E staining)
  • Measurement of pro-inflammatory mediators in liberated corneal cells (by flow cytometry or ELISA)

Porcine Cornea Reversibility Assay (PorCORA)

  • Uses excised porcine corneas that are normally discarded as waste in food production
  • Corneas maintained in culture for up to 21 Days
  • Measures "Days to Recover" after exposure to possible Irritants
  • Allows discrimination between Severe Irritant and Ocular Corrosive

Keratinocyte/Fibroblast Viability Assay (Neutral Red or MTT)


MB Research uses normal human primary epidermal keratinocytes or fibroblasts (or mouse keratinocytes and fibroblasts)
  • Cells are representative of the eye and skin epithelium
  • Human cells are derived from neonatal, adolescent, or aged donors
  • Cytokine expression or release (LDH, PGE-2, interleukins) can be measured
  • Low serum or serum free protocol options are available

Keratinocyte Proliferation (turnover) Assay

  • Using primary human cell or mouse cell lines in serum-free or low serum media
  • Rate of proliferation or apoptosis is measured by vital dye uptake (MTT or neutral red) or by BrdU incorporation and flow cytometry

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Dermal Irritation Assays

Three Dimensional Human Skin Equivalent Systems

The 3D models available at MB Research (MatTek EpiDerm™ and SkinEthic systems) allow the application of the test material directly to the "skin" surface and permit the assessment of dermal toxicity via a wide variety of parameters.

  • Cytotoxicity via MTT, neutral red viability assay, or flow cytometry
  • Histological examination (H & E) of the epidermal layers, including the stratum corneum
  • Measurement of inflammatory mediator and lytokine release (PGE-2, nitric oxide, TNFa, IL1a, and other interleukins)
  • Dissolution of the tissue equivalent into a single cell suspension, with subsequent flow cytometric analysis allows evaluation of gene expression and peroxide production (antioxidant status of cells)

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Dermal Corrosivity Assays


Corrositex™

  • An accepted alternative to the Draize rabbit corrosivity test that recently has been validated by ICCVAM for use for DOT, FHSA, CPSC, EPA and FDA testing
  • Allows Packing Group assignment of test materials
  • Can be used to compare the relative corrosivity of materials within Packing Groups for product development purposes. See www.corrositex.com for more information.

MatTek EpiDerm™ Corrosivity Test

  • Validated by ECVAM and ICCVAM as an in vitro alternative corrosivity test based on human skin cells
  • Allows testing of material not compatible with Corrositex™

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Phototoxicity Assays

  • With 3T3 mouse fibroblast cells via the EC/COLIPA validated protocol. See www.3t3nru.com for more information.
  • Using the MatTek EpiDerm™ protocol currently under review by ECVAM
  • Human keratinocytes or fibroblasts

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