MB Research Laboratories - Alternative Ocular Irritation Testing

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MB Research Labs
PO Box 178 Spinnerstown, PA 18968
voice: (215) 536-4110
fax: (215) 536-1816

Chorioallantoic Membrane Vascular Assay
Alternative Ocular Irritation

Day 7 - Vascular development of the Chorioallantoic Membrane

ASSAY HIGHLIGHTS:

Non-animal alternative to Draize eye test
Uses the vascularized chorioallantoic membrane
High concordance to in vivo data

OVERVIEW:
Damage to the vascular structure (i.e. ghost vessels, capillary injection, and/or hemorrhaging) of the chorioallantoic membrane (CAM) of fertilized chicken eggs is assessed after topical exposure of a test chemical which is then used as an endpoint to determine the potential for conjunctival irritancy.

APPLICATIONS:
CAMVA can be used to investigate the vascular effects such as neovascularization, angiogenesis, and anti-angiogenesis, which are of particular interest to pharmaceutical and cosmetic product research and development.

The chorioallantoic membrane is evaluated for vascular damage, such as hemorraghes, capillary injection, loss of blood flow or ghost vessels, angiogenesis and antiangiogenesis.