Delayed Contact Dermal Sensitization Test - Buehler Method

Objective:

To determine the potential of a product to promote skin sensitization reactions after repeated applications using the method of Ritz & Buehler, 1980, Current Concepts in Cutaneous Toxicity. This study is designed to comply with the standards set forth in EPA Health Effects Test Guidelines, OCSPP 870.2600, final guideline, March 2003. OECD Guideline 406 is referred to in OCSPP 870.2600 as an acceptable method to assess the potential of a product to promote skin sensitization reaction.

What are In Vivo Sensitization Tests?

Test methods designed to identify substances with skin sensitization potential or cause allergic contact dermatitis, which is an important element in evaluating toxicity. Below are the available in vivo tests for dermal sensitization available:

In Vivo Sensitization Testing Assays