Genotoxicology Assays

Bacterial Reverse Mutation Assay (Ames Assay)

The purpose of this study is to evaluate the mutagenic potential of a test article based on the reversion of selective growth mutations in several strains of Salmonella typhimurium bacteria and in Escherichia coli WP2 uvrA bacteria, in the presence and absence of S9 activation.

This protocol is based on:

OECD Guideline for Testing of Chemicals: No. 471 – Bacterial Reverse Mutation Test (July 1997) and U.S. EPA Health Effects Test Guidelines OPPTS 870.5100 – Bacterial Reverse mutation Test (August 1998).
Bacterial Reverse Mutation Assay

Basis of the Method:

The test system is exposed to a test article via the plate incorporation method, which has been shown to effectively detect a wide variety of mutagenic compounds. This assay is based on the methodology originally described by Ames, et al. (1975) and updated by Maron and Ames (1983).

Assay Points:

  • Plate Incorporation Method
  • Five Strains Tested per Assay (WP2, TA97a, TA1535, TA98, TA100)
  • Five Concentrations per Test Article
  • Triplicate Plates per Concentration
  • Toxicity Screen (One Strain, 5 Concentrations)
  • Validation Performed on 12 Chemicals