Innovation
MB Research Labs conducts Good Laboratory Practice (GLP) compliant toxicology assays as well as low-cost screening studies for clients in the cosmetic, consumer product, chemical, biotech and pharmaceutical industries.In Vitro and Alternative
1) Three Dimensional Human Ocular Tissue Equivalent Systems
- MatTek EpiOcular™, SkinEthic corneocyte models
- These models mimic characteristics of the epithelium of the eye
- Unique properties of 3D models allow test articles to be applied topically, allowing testing of solids, organics, and insoluble materials
2) Porcine Cornea Reversibility Assay (PorCORA)
- Draize Ocular Irritation Alternative
- Measures recovery of corneal epitheilum after topical dosing to discriminate between severe irritation or ocular corrosivity
- See ROBatt- proposed tiered testing strategy for Draize Rabbit Eye Test Replacement.
3) Chorioallantoic Membrane Vascular Assay (CAMVA)
- Uses fertilized chicken eggs on embryonic day 10 or 14 of development
- Measures hemodynamic effects, injury, and anti-angiogenic effects to the membrane microvasculature
- Especially useful for alcohol-containing formulations
- See ROBatt- proposed tiered testing strategy for Draize Rabbit Eye Test Replacement.
4) Hen's Egg Test-Chorioallantoic Membrane (HET-CAM)
- Draize Rabbit Eye Test Alternative
- Uses fertilized chicken eggs on embryonic day 10 or 14 of development
- Measures hemodynamic effects, injury, and anti-angiogenic effects to the membrane microvasculature
- Especially useful for alcohol-containing formulations
5) Bovine Corneal Opacity and Permeability Assay (BCOP)
- Draize Rabbit Eye Test Alternative
- Uses excised bovine corneas normally discarded
- Measures two endpoints; 1) changes in opacity, reflecting protein denaturation and corneal injury, and 2) fluorescein permeability reflecting damage to corneal epithelium
- Optional histological endpoint is available (H & E staining)
- Measurement of pro-inflammatory mediators in liberated corneal cells (by flow cytometry or ELISA)
- See ROBatt- proposed tiered testing strategy for Draize Rabbit Eye Test Replacement.
6) Replacement Ocular Battery (ROBatt)
- Tiered Testing Strategy aimed to replace Draize Rabbit Eye Test
- Uses suite of ocular irritation alternative test methods to effectively screen test materials and classify them in accordance to existing regulatory classes (GHS, EPA, EU)
Chorioallantoic Membrane of fertilized chicken egg used to assess potential ocular irritation of compounds and chemicals.
MB has developed PorCORA - Porcine Cornea Reversibility Assay. An alternative to the Draize Rabbit Eye Test that is capable of determining time to reversal after exposure to an ocular irritant.
MatTek's EpiOcular and EpiDerm are used for screening of test materials using a 3D human tissue construct.