Alternative & In Vitro Ocular Irritation Assays

MB Research Labs conducts Good Laboratory Practice (GLP) compliant in vitro toxicology assays as well as low-cost screening studies for clients in the cosmetic, consumer product, chemical, biotech and pharmaceutical industries.

NEW - OptiSafe™ Non-Animal Ocular Irritation Test

  • OptiSafe™
  • An in vitro test method in which a test substance is applied to a semi-permeable membrane. Damage to macromolecules in the membrane is measured to assess the test substance‚Äôs potential to cause eye irritation.
  • Can be used to determine the irritation potential of cosmetics, creams, and a wide variety of consumer products. Results are presented as GHS, EPA classifications, an ocular irritation score and class.

Three Dimensional Human Ocular Tissue Equivalent Systems

  • MatTek EpiOcular™, SkinEthic corneocyte models
  • These models mimic characteristics of the epithelium of the eye
  • Unique properties of 3D models allow test articles to be applied topically, allowing testing of solids, organics, and insoluble materials

Bovine Corneal Opacity and Permeability Assay (BCOP)

  • Draize Rabbit Eye Test Alternative
  • Uses excised bovine corneas normally discarded
  • Measures two endpoints; 1) changes in opacity, reflecting protein denaturation and corneal injury, and 2) fluorescent dye permeability reflecting damage to corneal epithelium
  • Optional histological endpoint is available (H & E staining)
  • Measurement of pro-inflammatory mediators in liberated corneal cells (by flow cytometry or ELISA)
  • See ROBatt- proposed tiered testing strategy for Draize Rabbit Eye Test Replacement.

Hen's Egg Test-Chorioallantoic Membrane (HET-CAM)

  • Draize Rabbit Eye Test Alternative
  • Uses fertilized chicken eggs on embryonic day 10 or 14 of development
  • Measures hemodynamic effects, injury, and anti-angiogenic effects to the membrane microvasculature
  • Especially useful for alcohol-containing formulations

Chorioallantoic Membrane Vascular Assay (CAMVA)

  • Uses fertilized chicken eggs on embryonic day 10 or 14 of development
  • Measures hemodynamic effects, injury, and anti-angiogenic effects to the membrane microvasculature
  • Especially useful for alcohol-containing formulations
  • See ROBatt- proposed tiered testing strategy for Draize Rabbit Eye Test Replacement.

Porcine Cornea Reversibility Assay (PorCORA)

  • Draize Ocular Irritation Alternative
  • Measures recovery of corneal epitheilum after topical dosing to discriminate between severe irritation or ocular corrosivity
  • See ROBatt- proposed tiered testing strategy for Draize Rabbit Eye Test Replacement.

Replacement Ocular Battery (ROBatt)

  • Tiered Testing Strategy aimed to replace Draize Rabbit Eye Test
  • Uses suite of ocular irritation alternative test methods to effectively screen test materials and classify them in accordance to existing regulatory classes (GHS, EPA, EU)