3Rs - Refine, Reduce, Replace

In Vitro & Alternative Test Methods

Since the late 1980s, MB Research has been a pioneer in the use and development of in vitro & alternative test methods, reducing the use of live animals in toxicological research.

What are the 3 Rs?

The principle of the 3Rs was first mentioned by William Russell and Rex Burch in 1959. In their book "The Principles of Humane Experimental Technique", they classified humane techniques under the headings Refinement, Reduction, and Replacement, commonly known now as the 3Rs.

MB Research Laboratories prides itself on pursuing in vitro and alternative toxicological methods that Reduce, Refine and Replace the use of animals in laboratory research and has worked with many leaders in this field to advance the development, validation and regulatory acceptance of in vitro and alternative tests with a goal on replacing laboratory animals in toxicological research.

Our 3Rs Impact

Currently, MB Research offers a wide variety of in vitro and alternative toxicity tests, such as the Local Lymph Node Assay (LLNA), the 3T3 Neutral Red Uptake Phototoxicity Assay (3T3 NRU Phototox), the Bovine Corneal Opacity and Permeability Assay (BCOP), the Chorioallantoic Membrane Vascular Assay (CAMVA), MatTek EpiDerm™, EpiOcular and MelanoDerm™ Assays.

MB Research is also conducting developmental and validation studies to advance application of in vitro and alternative toxicology tests. More information about currently funded research can be found here.